Thermo Fisher Scientific CD284 (TLR4) Monoclonal Antibody (HTA125), Alexa Fluor 700, eBioscience
인간 TLR4(CD284)을 인식하는 HTA125 단클론 항체로 Alexa Fluor 700으로 표지되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 인간 및 상어 시료에서 검증되었습니다. 선천면역 연구 및 TLR4 신호전달 분석에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 3 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 |
| Immunocytochemistry (ICC/IF) | - | 2 |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test | 14 |
| ELISA | - | 1 |
| Neutralization | - | 8 |
| Functional Assay | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human, Shark |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HTA125 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_10596522 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). TLR4, a member of the Toll family, is a type I transmembrane protein with leucine-rich repeats and a cytoplasmic domain homologous to the IL-1 receptor. It forms a complex with MD-2 and CD14, mediating LPS recognition and signaling in innate immunity and inflammation. TLR4 is expressed on peripheral blood monocytes.
HTA125 immunoprecipitates human TLR4 (~100 kDa) from transfected cells. TLR surface expression varies among donors and is generally low on monocytes and other immune cells. For optimal staining, it is recommended to use the lysed whole blood protocol rather than Ficoll-gradient prepared cells, as the latter may reduce staining intensity.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated for flow cytometry at 5 µL (1.0 µg) per test (100 µL final volume). Suitable for 10⁵–10⁸ cells/test.
Fluorochrome Information:
Alexa Fluor™ 700 emits at 723 nm, excited by the red laser (633 nm). Recommended filter: 685 LP mirror, 710/20 filter.
Excitation: 633–647 nm; Emission: 723 nm; Laser: Red.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TLR4 (Toll-like receptor 4) plays a key role in pathogen recognition and activation of innate immunity. It is expressed in placenta and myelomonocytic leukocytes, responding to LPS via a receptor complex with CD14 and LY96. TLR4 mediates cytokine production essential for immune responses. Mutations in TLR4 influence LPS responsiveness. Multiple transcript variants exist, though their protein-coding potential is uncertain. TLR4-deficient mice show increased susceptibility to Gram-negative bacterial infection.
For Research Use Only. Not for use in diagnostic procedures.
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