Thermo Fisher Scientific TIGIT Monoclonal Antibody (MBSA43), Alexa Fluor 700, eBioscience

Applications

Immunohistochemistry (IHC)

• Tested Dilution: Not specified
• View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.25 µg)/test
• View 14 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• Alexa Fluor 647
• Alexa Fluor 660
• APC
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 700
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The MBSA43 monoclonal antibody reacts with human TIGIT (T cell immunoreceptor with Ig and ITIM domains), also known as VSTM3 or WUCAM. This 26 kDa protein belongs to the poliovirus receptor (PVR) family. TIGIT is expressed on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but not on B cells or naive CD4+ T cells. Expression may be upregulated upon activation.

MBSA43 is reported to cross-react with Rhesus macaque.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells. Use 5 µL (0.25 µg) per test in 100 µL final volume. Suitable for 10⁵–10⁸ cells/test.

Fluorochrome Information:
Alexa Fluor 700 emits at 723 nm and is excited by the red laser (633–647 nm). Recommended filters: 685 LP mirror and 710/20 band pass filter.

Excitation: 633–647 nm
Emission: 723 nm
Laser: Red Laser

Target Information

TIGIT binds with high affinity to the poliovirus receptor (PVR), leading to increased IL10 and decreased IL12B secretion, thereby suppressing T cell activation and promoting the generation of mature immunoregulatory dendritic cells.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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