Thermo Fisher Scientific Phospho-Histone H2B (Ser36) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-Histone H2B (Ser-36) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-36 in human histone H2B. This sequence is well conserved in most isoforms of rat and mouse histone H2B, and has low homology to other histone family members.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942935

Product Specific Information

The antibody detects a 15 kDa* protein corresponding to the molecular mass of Histone H2B on SDS-PAGE immunoblots of human Jurkat cells treated with Calyculin A, and this reactivity is reduced after lambda phosphatase treatment.

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.