Thermo Fisher Scientific Eotaxin 3 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

ELISA (ELISA)

0.5-2.0 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human Eotaxin-3 (CCL26) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Eotaxin 3,uniProtId:Q9Y258-1,ncbiNodeId:9606,antigenRange:24-94,antigenLength:94,antigenImageFileName:500-P156G-100UG_Eotaxin_3_Q9Y258-1_Goat.svg,antigenImageFileNamePDP:500-P156G-100UG_Eotaxin_3_Q9Y258-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929528

Product Specific Information

AA Sequence of recombinant protein: TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Human Eotaxin-3 (CCL26). Anti-Human Eotaxin-3 (CCL26)-specific antibody was purified by affinity chromatography employing an immobilized Human Eotaxin-3 (CCL26) matrix.

Sandwich ELISA: To detect Human Eotaxin-3 (CCL26) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Eotaxin-3 (CCL26) (500-P156GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Eotaxin-3 (CCL26).

Western Blot: To detect Human Eotaxin-3 (CCL26) by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Eotaxin-3 (CCL26) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Eotaxin-3 is a small cytokine belonging to the CC chemokine family that is also called CCL26, Chemokine (C-C motif) ligand 26. Chemokines play pivotal roles in health and disease by controlling migration and tissue localization of specific types of cells expressing their cognate receptors. Eotaxin-3 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3. It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimulated with the cytokine interleukin 4. This gene for chemokine is located on human chromosome 7. Eotaxin-3 induced migration of eosinophils and basophils only at a 10-fold higher concentration than eotaxin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.