Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

CHO cells-derived Recombinant Murine IL-12 p70 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-12 p70,uniProtId:P43431-1,ncbiNodeId:10090,antigenRange:23-215,antigenLength:215,antigenImageFileName:500-P155GBT-500UG_IL-12_p70_P43431-1_Goat.svg,antigenImageFileNamePDP:500-P155GBT-500UG_IL-12_p70_P43431-1_Goat_PDP.jpeg,sortOrder:3},{targetFamily:IL-12 p70,uniProtId:P43432-1,ncbiNodeId:10090,antigenRange:23-335,antigenLength:335,antigenImageFileName:500-P155GBT-500UG_IL-12_p70_P43432-1_Goat.svg,antigenImageFileNamePDP:500-P155GBT-500UG_IL-12_p70_P43432-1_Goat_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AA Sequence of recombinant protein: p35 subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine IL-12. Anti-Murine IL-12-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect mIL-12 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-12 (500-P155G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mIL-12.

Western Blot: To detect mIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mIL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P155GBT-1MG will be provided as 2 x 500 µg

Target Information

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system`s response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.