Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein
p35 subunit:
RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA

p40 subunit:
MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS

Preparation
Produced from sera of goats immunized with highly pure Recombinant Murine IL-12. Anti-Murine IL-12-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA
To detect mIL-12 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-12 (500-P155G) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant mIL-12.

Western Blot
To detect mIL-12 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant mIL-12 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information
500-P155GBT-1MG will be provided as 2 × 500 µg.

Target Information

IL-12 p70 (Interleukin-12) is a cytokine critical for immune responses against infections and cancer. It is produced by dendritic cells, macrophages, and B cells in response to pathogens and tumor cells. IL-12 p70 induces interferon-gamma production, activating T cells and NK cells. It is a potential therapeutic target for infectious diseases, autoimmune disorders, and cancer, enhancing immune responses and modulating inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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