Thermo Fisher Scientific Eotaxin 3 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human Eotaxin-3 (CCL26) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Eotaxin 3,uniProtId:Q9Y258-1,ncbiNodeId:9606,antigenRange:24-94,antigenLength:94,antigenImageFileName:500-P156GBT-500UG_Eotaxin_3_Q9Y258-1_Goat.svg,antigenImageFileNamePDP:500-P156GBT-500UG_Eotaxin_3_Q9Y258-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AA Sequence of recombinant protein: TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Human Eotaxin-3 (CCL26). Anti-hEotaxin-3 (CCL26)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hEotaxin-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Eotaxin-3 (500-P156G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEotaxin-3.

Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEotaxin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P156GBT-1MG will be provided as 2 x 500 µg

Target Information

Eotaxin-3 is a small cytokine belonging to the CC chemokine family that is also called CCL26, Chemokine (C-C motif) ligand 26. Chemokines play pivotal roles in health and disease by controlling migration and tissue localization of specific types of cells expressing their cognate receptors. Eotaxin-3 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3. It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimulated with the cytokine interleukin 4. This gene for chemokine is located on human chromosome 7. Eotaxin-3 induced migration of eosinophils and basophils only at a 10-fold higher concentration than eotaxin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.