Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, Biotin, PeproTech
마우스 IL-12 p70 인식용 바이오틴 결합 폴리클로날 항체로, ELISA와 Western Blot에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도 재조합 단백질로 면역화된 염소 혈청에서 생산되었습니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific IL-12 p70 Polyclonal Antibody, Biotin (PeproTech)
Applications and Tested Dilution
- Western Blot (WB): 0.1–0.2 µg/mL
- ELISA: 0.25–1.0 µg/mL
- Sandwich ELISA 시, PeproTech Polyclonal Anti-Murine IL-12 (500-P155G)와 함께 사용하여 0.2–0.4 ng/well 검출 가능
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHO cells-derived Recombinant Murine IL-12 p70 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929526 |
Product Specific Information
AA Sequence of recombinant protein:
- p35 subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA
- p40 subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine IL-12. Anti-Murine IL-12-specific antibody was purified by affinity chromatography and then biotinylated.
Western Blot:
To detect mIL-12 by Western Blot analysis, use at 0.1–0.2 µg/mL. Detection limit for recombinant mIL-12: 1.5–3.0 ng/lane (reducing or non-reducing conditions).
Packaging:
500-P155GBT-1MG is provided as 2 × 500 µg.
Target Information
IL-12 p70 (Interleukin-12) is a cytokine critical to immune response against infections and cancer. It is produced by dendritic cells, macrophages, and B cells, stimulating interferon-gamma production and T/NK cell activation. IL-12 p70 is a key target in research on autoimmune and infectious diseases, as well as cancer immunotherapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: 500-P155GBT-25UG_IL-12_p70_P43431-1_Goat.svg, 500-P155GBT-25UG_IL-12_p70_P43431-1_Goat_PDP.jpeg, 500-P155GBT-25UG_IL-12_p70_P43432-1_Goat.svg, 500-P155GBT-25UG_IL-12_p70_P43432-1_Goat_PDP.jpeg)
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