Thermo Fisher Scientific HDAC9 Monoclonal Antibody (OTI1H4), TrueMAB

Applications

• Western Blot (WB)
  Tested Dilution: 1:2,000

Product Specifications

Target Information

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. Chromatin remodeling is a critical component of transcriptional regulation, and acetylation of nucleosomal histones is a major source of this remodeling. Acetylation of lysine residues in the amino-terminal tail domain of histone leads to an allosteric change in nucleosomal conformation and increased accessibility to transcription factors.

Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Conversely, deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1–9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity, and repress myocyte enhancer-binding factor 2-mediated transcription. HDAC9 and HDAC9a are expressed in brain, skeletal muscle, kidney, placenta, and pancreas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.