Thermo Fisher Scientific p53 Monoclonal Antibody (OTI6C3), TrueMAB

Applications

• Western Blot (WB)

Tested Dilution: 1:2,000

Product Specifications

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. Upon activation, p53 induces transcription of genes such as p21waf1, leading to G1 or G2/M arrest. Structurally, p53 includes an N-terminal transactivation domain, a central DNA-binding domain, an oligomerization domain, and a C-terminal regulatory domain.

Phosphorylation at Ser15 is critical for activation and stabilization, while phosphorylation at Ser392 is associated with tumor formation. p53 plays a key role in preventing proliferation of damaged cells, acting as an anticancer factor. It is also linked to aging, oxidative stress, and cognitive functions such as long-term potentiation. Normal cells have low p53 expression, whereas transformed cell lines show high expression, contributing to malignancy. Mutations in the TP53 gene can abolish DNA binding and tumor suppressor activity, and germline mutations are associated with Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.