Thermo Fisher Scientific Phospho-cdc25C (Thr48) Polyclonal Antibody
Phospho-cdc25C (Thr48) 폴리클로날 항체로, 인간과 생쥐 시료에서 인산화된 CDC25C(Thr48) 단백질을 특이적으로 검출합니다. Western blot 및 IHC(P) 적용 가능하며, 고순도의 rabbit IgG 항체입니다. 세포 주기 조절 연구용으로 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CDC25C (Accession P30307), corresponding to amino acid residues around phosphorylated Thr48 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816988 |
Product Specific Information
This antibody detects endogenous levels of CDC25C only when phosphorylated at Thr48.
Target Information
M-phase inducer phosphatase 3 is an enzyme encoded by the CDC25C gene in humans. This gene is highly conserved and plays a key role in cell division regulation. The encoded protein is a tyrosine phosphatase belonging to the Cdc25 phosphatase family. It dephosphorylates cyclin B-bound CDC2 (CDK1), triggering entry into mitosis, and may suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants have been reported, though many are not fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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