AAVpro Helper Free Systems (AAV-CRE Recombinase) are kits for the preparation of adeno-associated virus (AAV) particles to deliver a Cre recombinase gene cassette to mammalian cells. Cre recombinase is a bacterial protein that recognizes a specific 34-bp sequence (loxP site) containing an 8-bp core sequence where recombination takes place. The orientation and location of introduced loxP sites allow for Cre-mediated gene inversion, translocation, or deletion.
AAVpro Helper Free Systems (AAV-CRE Recombinase) are kits for the preparation of adeno-associated virus (AAV) particles to deliver a Cre recombinase gene cassette to mammalian cells. Cre recombinase is a bacterial protein that recognizes a specific 34-bp sequence (loxP site) containing an 8-bp core sequence where recombination takes place. The orientation and location of introduced loxP sites allow for Cre-mediated gene inversion, translocation, or deletion. With these kits, high-titer AAV particles can be prepared without a helper virus by simply transfecting AAVpro 293T cells with the three included plasmids that encode all of the factors necessary to prepare recombinant AAV particles. AAV Extraction Solution is also included for efficient AAV particle isolation.
More Less
