
Takara Edman degradation and protein sequencing: Pfu Methionine Aminopeptidase
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More Information
Applications
- Release of N-terminal methionine residues from recombinant proteins and peptides
Source
Escherichia coli transformed with Pyrococcus furiosus methionine aminopeptidase gene.
Storage
–20°C
Properties
- Molecular weight: 32.848 (MS analysis), 37.000 (SDS-PAGE)
- Optimum temperature: 85–95°C
- Thermostability: Stable for 1 hr at 75°C, pH 7.2 and 0.5 mM Co2+
- Optimum pH: 7.0–8.0
- Tolerance to denaturants: ≤2 M Urea, ≤0.2 M Guanidine-HCl, ≤0.01% SDS
- Activator: Co2+
- Inhibitor: EDTA
Form
Solution of 10 mM Tris-HCl, pH 7.5, containing 0.01% Tween 20 and 0.1 mM CoCl2
Product citations
Ben-Bassat, A. et al. Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure. J. Bacteriol. *169, *751–757 (1987).
Ben-Bassat, A. et al. Amino-terminal processing of proteins. Nature *326, *315 (1987).
Miller, C.G. et al. N-terminal methionine-specific peptidase in Salmonella typhimurium. Proc. Natl. Acad. Sci. USA 84,2718–2722 (1987).
Yasueda, H. et al. High-level direct expression of semi-synthetic human interleukin-6 in Escherichia coli and production of N-terminus met-free product. Biotechnology 8,1036–1040 (1990).
Additional product information
Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
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