Takara Edman degradation and protein sequencing: Pfu Methionine Aminopeptidase

Applications

• Release of N-terminal methionine residues from recombinant proteins and peptides

Source

Escherichia coli transformed with Pyrococcus furiosus methionine aminopeptidase gene.

Storage

-20°C

Properties

Form

Solution of 10 mM Tris-HCl, pH 7.5, containing 0.01% Tween 20 and 0.1 mM CoCl2

Product Citations

• Ben-Bassat, A. et al. Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure. J. Bacteriol. 169, 751–757 (1987).
• Ben-Bassat, A. et al. Amino-terminal processing of proteins. Nature 326, 315 (1987).
• Miller, C.G. et al. N-terminal methionine-specific peptidase in Salmonella typhimurium. Proc. Natl. Acad. Sci. USA 84, 2718–2722 (1987).
• Yasueda, H. et al. High-level direct expression of semi-synthetic human interleukin-6 in Escherichia coli and production of N-terminus met-free product. Biotechnology 8, 1036–1040 (1990).

Additional Product Information

Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
See the Kit Components List for included items.
Certificates of Analysis and Kit Components Lists are available under the Documents tab.

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