Thermo Fisher Scientific Phospho-Histone H2A.X (Ser139) Recombinant Rabbit Monoclonal Antibody (SR33-09)
Western blot, IHC, ICC에 최적화된 인산화 히스톤 H2A.X (Ser139) 특이 항체. 재조합 토끼 단클론으로 높은 특이성과 재현성 제공. Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도, 냉장 또는 냉동 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1000–1:2000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:5000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SR33-09 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser139 of Human Histone H2AX |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809284 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences are cloned from immunoreactive rabbits, and individual clones are screened to select the best candidates.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to the large rabbit immune repertoire
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome core particle.
Phosphorylation of serine 139 at the C-terminus of H2AX (γ-H2AX) occurs in response to DNA double-strand breaks (DSBs) and can be detected by Western blot or immunofluorescence.
Phosphorylation is mediated by phosphatidylinositol 3-kinases, possibly ATM, DNA-PK, or ATR.
H2A.X consists of 143 amino acids and functions as a basal histone synthesized in both G1 and S-phase. Two mRNA forms exist: one polyadenylated (~1600 bases) and one non-polyadenylated (~575 bases).
The gene maps to chromosome 11q23.2–q23.3 in humans and plays a key role in chromatin structure and DNA repair signaling through MDC1 recruitment.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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