Thermo Fisher Scientific HSP90 alpha/beta Monoclonal Antibody (Hyb-K41220A), FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat, Yeast

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

Hyb-K41220A

Immunogen

Recombinant human HSP90alpha; Specificity mapped to amino acids 291-304 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSP90,uniProtId:P08238-1,ncbiNodeId:9606,antigenRange:291-304,antigenLength:724,antigenImageFileName:MA5-45155_HSP90_P08238-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45155_HSP90_P08238-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.09mM sodium bicarbonate/PBS, pH 7.2, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931609

Product Specific Information

1 µg/mL was sufficient for detection of HSP90 alpha beta in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects 90kDa. Will detect both alpha (inducible) and beta (constitutively-expressed) forms.

Target Information

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 and mitochondria (Chen et al., 2005 ).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.