Thermo Fisher Scientific HO-1 Monoclonal Antibody (1F12-A6), FITC
HO-1 단백질을 인식하는 FITC 결합 마우스 단클론 항체로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 사용 가능. 사람 및 다종 교차 반응성 보유. 단백질 G 정제, 4°C 암소 보관. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F12-A6 |
| Immunogen | Human HO-1 synthetic peptide, amino acids 1–30 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.2, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931607 |
Additional Formats
- APC (Cat. MA5-45152)
- PerCP (Cat. MA5-45154)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL was sufficient for detection of HO-1 in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary. Detects 32 kDa. Does not cross-react with HO-2.
Target Information
Heme oxygenase (HO) is a microsomal enzyme catalyzing the oxidation of heme to biliverdin and carbon monoxide. Two isozymes exist: inducible HO-1 and constitutive HO-2. HO-1 expression increases under oxidative stress, inflammation, TGF-β stimulation, and heat shock, functioning as a cellular defense mechanism by generating antioxidant bilirubin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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