Thermo Fisher Scientific HO-1 Monoclonal Antibody (1F12-A6), PerCP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1F12-A6

Immunogen

Human HO-1 synthetic peptide, amino acids 1-30 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HO-1,uniProtId:P09601-1,ncbiNodeId:9606,antigenRange:1-30,antigenLength:288,antigenImageFileName:MA5-45154_HO-1_P09601-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45154_HO-1_P09601-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PerCP PerCP PerCP

View additional formats

• APC
• FITC
• Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.2, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931608

Product Specific Information

1 µg/mL was sufficient for detection of HO-1 in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects 32kDa. Does not cross-react with HO-2.

Target Information

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.