Thermo Fisher Scientific HO-1 Monoclonal Antibody (1F12-A6), PerCP
HO-1 단백질을 검출하기 위한 PerCP 결합 단클론 항체. Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. Human 등 다양한 종에 반응. 단백질 G로 정제된 액상 형태로, 4°C 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F12-A6 |
| Immunogen | Human HO-1 synthetic peptide, amino acids 1–30 |
| Conjugate | PerCP |
| Excitation/Emission Max | 482/675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931608 |
Available Formats
Product Specific Information
1 µg/mL was sufficient for detection of HO-1 in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Detects 32 kDa. Does not cross-react with HO-2.
Target Information
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules biliverdin and carbon monoxide. HO consists of two homologous isozymes: an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by various stimuli including oxidative stress, inflammatory agents, transforming growth factor beta, and heat shock. The increase in expression of HO-1 is considered a cellular defense mechanism against oxidative stress, as elevated HO can generate more bilirubin, an antioxidant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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