Thermo Fisher Scientific HSP90 beta Monoclonal Antibody (Hyb-K3701), FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:3,000

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

Hyb-K3701

Immunogen

Recombinant human HSP90beta; Specificity mapped to amino acids 185-335 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSP90 beta,uniProtId:P08238-1,ncbiNodeId:9606,antigenRange:185-335,antigenLength:724,antigenImageFileName:MA5-45156_HSP90_beta_P08238-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45156_HSP90_beta_P08238-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

1 µg/mL was sufficient for detection of HSP90 beta in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects 90kDa. This is a beta specific product, does not cross-react with alpha isoforms.

Target Information

Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and some kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.