Thermo Fisher Scientific ER-beta (Estrogen Receptor beta-2) Monoclonal Antibody (57/3)
ER-beta2 이소폼에 특이적인 단클론 항체로, Western blot, IHC, ICC, Flow Cytometry 등에 사용 가능. 사람 시료에 반응하며 교차 반응 없음. Protein A/G로 정제된 액상 항체로, 4°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL |
| Flow Cytometry (Flow) | 2–4 µg/10^6 cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 57/3 |
| Immunogen | A synthetic peptide (CGMKMETLLPEATMEQ) from the C-terminus of the human estrogen receptor beta 2 isoform |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
This monoclonal antibody recognizes the estrogen receptor beta 2 (ERβ2) isoform, which is expressed in the nuclei of various human tissues including ovary, placenta, testis, and vas deferens. It shows no cross-reactivity with the estrogen receptor beta 1 isoform.
The ERβ2 gene encodes a member of the estrogen receptor family and the nuclear receptor transcription factor superfamily. The encoded protein contains an N-terminal DNA-binding domain and a C-terminal ligand-binding domain, and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17β-estradiol or related ligands, the protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms may dominantly inhibit the activity of other estrogen receptor family members.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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