Thermo Fisher Scientific ER-beta-1 (Estrogen Receptor beta-1) Monoclonal Antibody (ERb455)
Human ER-beta-1 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC/IF, Flow cytometry에 적합합니다. Unconjugated 형식이며 Protein A/G로 정제되어 있습니다. 4°C에서 보관하며 연구용으로만 사용됩니다.
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Thermo Fisher Scientific ER-beta-1 (Estrogen Receptor beta-1) Monoclonal Antibody (ERb455)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ERb455 |
| Immunogen | C-terminus fragment of recombinant human estrogen receptor beta protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- CF 488 conjugate
- PE conjugate
- Custom conjugation available upon request
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Estrogen Receptor beta (ER beta, NR3A2) is approximately 55 kDa, with multiple isoforms ranging from 36–59 kDa. Initially cloned from human testis, ER beta belongs to the nuclear receptor superfamily.
It functions as a ligand-activated transcription factor that binds estrogen and regulates gene expression through estrogen response elements (EREs).
Two receptor forms exist—ER alpha (ESR1) and ER beta (ESR2)—both binding estrogen with similar affinity.
ER beta isoforms can inhibit ER alpha activity in reproductive tissues and act as tumor suppressors in various cancers and diseases, including prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease, and cardiovascular disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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