
Thermo Fisher Scientific ER-beta (Estrogen Receptor beta-5) Monoclonal Antibody (PPG5/25)
ER-beta (Estrogen Receptor beta-5) 단일클론 항체로, 인간 시료에 반응하며 WB, IHC, ICC 등 다양한 응용에 적합합니다. PPG5/25 클론으로 높은 특이성과 재현성을 제공하며, 단백질 A/G 정제 및 PBS 버퍼로 안정화되어 있습니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PPG5/25 |
| Immunogen | Tuberculin conjugated synthetic peptide (LLSHVRHARYAP) derived from the C-terminus of human Estrogen Receptor beta 5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
This monoclonal antibody specifically recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ER beta (ESR2), which exists in at least five isoforms (ERB1–ERB5). No cross-reactivity is observed with estrogen receptor beta 1 or 2 isoforms.
The encoded protein is part of the estrogen receptor family and the nuclear receptor transcription factor superfamily. It contains N-terminal DNA binding and C-terminal ligand binding domains, localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17β-estradiol or related ligands, the protein forms homo- or heterodimers that interact with specific DNA sequences to activate transcription. Some isoforms can dominantly inhibit other estrogen receptor family members.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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