Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Alexa Fluor 488, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 5 publications

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532, 561, 647, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 563, 737, 805
• Brilliant Violet 421, 711, 786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue / Red / UV / Violet / Yellow Series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha).
CD127 forms a complex with CD132 (common gamma chain) to create the functional IL-7 receptor (IL-7R).
It is a type I glycoprotein (75–80 kDa) expressed on immature B cells, thymocytes, and most mature T cells, with transient down-regulation upon activation.

IL-7 binding triggers signal transduction via tyrosine kinase pathways, including Jak/STAT.
IL-7 is essential for lymphocyte development and homeostatic proliferation of peripheral T cells.
IL-7R signaling also regulates TCR locus rearrangement in γδ T cells.

Recent studies show that CD127 expression is down-regulated on CD4⁺CD25⁺ regulatory T cells (Tregs).
CD4⁺CD25⁺CD127⁻ cells are more highly enriched for Tregs, expressing Foxp3 and exhibiting suppressive activity in vitro.

Binding of eBioRDR5 to PBMCs is blocked by pre-incubation with recombinant human IL-7 (Product #14-1079-80).

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells.
Use 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Excitation: 488 nm Emission: 519 nm Laser: Blue Laser

Target Information

CD127 (Interleukin-7 receptor, IL-7R) is a glycoprotein regulating lymphopoiesis.
The active receptor is an alpha/gamma chain heterodimer (IL7Rα/γc).
The γc chain activates signal transduction, while the α chain mediates specific signaling through cytoplasmic molecules.

CD127 supports proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4⁺/CD8⁺ T cells.
IL-7 binding to CD127 mediates critical immune functions.
Defects in CD127 are associated with severe combined immunodeficiency (SCID).

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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