Thermo Fisher Scientific CD27 Monoclonal Antibody (OTI1D4D1)

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily, which includes CD40 and CD30. Members of this family regulate cell proliferation and death.
Expression of CD27 and its ligand CD70 is mainly confined to lymphocytes. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells, and a subset of B cells.
Activation of T cells via the TCR-CD3 complex increases CD27 expression and leads to the release of soluble CD27 (sCD27), detectable in plasma, urine, or spinal fluid. Soluble CD27 serves as a prognostic marker for B cell malignancies.
RgpA, a cysteine proteinase, can degrade CD27 and counteract T cell activation mediated by CD27/CD70. CD27-binding protein (SIVA) is a proapoptotic protein that binds to CD27 and may mediate apoptosis via this receptor.
Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.