Thermo Fisher Scientific CD27 Monoclonal Antibody (OTI5G2E2)

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Flow Cytometry (Flow)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to reach a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily, including CD40 and CD30.
It regulates cell proliferation and death and is mainly expressed in lymphocytes.
CD27 forms disulfide-linked homodimers on mature thymocytes, peripheral blood T cells, and some B cells.
Activation of T cells via TCR-CD3 increases CD27 expression and releases soluble CD27 (sCD27, 28–32 kDa) detectable in plasma, urine, or spinal fluid.
Soluble CD27 serves as a prognostic marker for B cell malignancies.
CD27-binding protein (SIVA) is a proapoptotic protein involved in CD27-mediated apoptosis.
Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.