Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), NovaFluor Yellow 610, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.2 µg)/test

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha is expressed on mast and basophil cells and is upregulated by the presence of IgE. It forms a tetrameric complex with one beta and two gamma subunits possessing ITAM motifs, playing a key role in IgE-mediated allergic reactions.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of peripheral blood cells. Recommended use: 5 µL (0.2 µg) per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
• Add 5 µL of CellBlox Plus Blocking Buffer per sample, regardless of the number of NovaFluors in the panel.
• For single-color controls, add 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates use Phiton™ technology based on nucleic acid dye structures engineered for optimized fluorescent signatures and reduced spillover/spread.

Excitation: 552 nm
Emission: 612 nm
Laser: 561 nm (Yellow)

Target Information

FceR1 (Fc epsilonR1 alpha):
A subunit of the high-affinity IgE receptor complex (αβγ₂). The alpha subunit binds IgE, while beta and gamma subunits mediate signal transduction. This receptor is central to allergic responses; cross-linking by allergen-bound IgE triggers release of histamine and other mediators, leading to inflammation typical of hay fever and asthma.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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