Thermo Fisher Scientific CD44v3 Monoclonal Antibody (VFF-327)
인간 CD44v3 항원을 인식하는 마우스 모노클로날 항체로, IHC(P/F) 및 Flow Cytometry에 사용 가능. 단백질 G로 정제된 액상 형태이며, 1 mg/mL 농도. 세포 표면 CD44 분석 및 암 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VFF-327 |
| Immunogen | Proprietary |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | <0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538439 |
Product Specific Information
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for staining of paraffin sections.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10^6 cells in 100 µL.
Target Information
CD44 cell surface antigen is a 100 kDa glycoprotein widely expressed on human leukocytes, brain white matter, and some epithelial cells of the intestine and breast.
Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues.
CD44 acts as a receptor for hyaluronic acid (HA) and participates in cell-cell interactions, adhesion, and migration.
It is also involved in lymphocyte activation, recirculation, and homing.
CD44 expression may be up-regulated in certain carcinomas, potentially associated with metastatic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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