Thermo Fisher Scientific CD44 v7-v8 Monoclonal Antibody (VFF-17)
CD44 v7-v8 단백질을 인식하는 Mouse monoclonal antibody. IHC(P), IHC(F), Flow Cytometry에 적합. Human 시료에 반응하며, 1 mg/mL 농도의 액상 형태로 제공. 세포 부착 및 이동 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | 1:10–1:100 |
| Miscellaneous PubMed (Misc) | – |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VFF-17 |
| Immunogen | Proprietary |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538438 |
Product Specific Information
- Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for staining of paraffin sections.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Target Information
CD44 cell surface antigen is a 100 kDa glycoprotein widely expressed on human leukocytes, white matter of the brain, and some epithelial cells of the intestine and breast.
Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues.
CD44 acts as a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, adhesion, and migration.
It also participates in lymphocyte activation, recirculation, and homing.
CD44 expression may be upregulated in some carcinomas, potentially related to metastatic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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