Takara iDimerize Inducible Homodimer System

Takara iDimerize Inducible Homodimer System

The iDimerize Inducible Homodimer System enables real-time, small-molecule control of protein-protein interactions. A protein of interest is fused to the DmrB binding domain, and dimerization is induced by adding the B/B Homodimerizer (identical to the AP20187 ligand). Both plasmid and lentiviral (Lenti-X) vector formats are available.

Key Features

• Real-time control of protein dimerization using a small-molecule ligand
• Compatible with plasmid and lentiviral vector formats
• Enables reversible and specific dimerization
• Suitable for both cytoplasmic and membrane proteins

Note

While it is possible to use the iDimerize Inducible Homodimer System to induce heterodimerization, this will result in a mixture of homodimers and heterodimers.
To induce heterodimers specifically and exclusively, use the iDimerize Inducible Heterodimer System.

iDimerize Inducible Homodimer Systems (with Tet-On 3G Technology)

Ligand-dependent dimerization experiments may show unwanted non-ligand-induced dimerization when the protein of interest is overexpressed, especially for membrane proteins where local concentration can increase rapidly.
By combining iDimerize and Tet-On 3G technologies, these unwanted events can be minimized.

1. Use doxycycline to adjust protein expression to physiologically relevant levels.
2. Induce dimerization by adding the dimerizer ligand to the culture medium.