Takara Secreted alkaline phosphatase assays

Overview

• One-step assay for monitoring promoter activity
• 10-fold higher sensitivity than firefly luciferase
• No-cell-lysis protocol
• Adaptable from single-tube to high-throughput assays
• Dual live-cell assay includes secreted luciferase as well as SEAP, allowing monitoring of two promoters or using one reporter as a normalization control

Applications

• Time-course studies
• Non-invasive reporter gene assays using secreted alkaline phosphatase
• Screening of multiple compounds
• Perform downstream experiments with the same cells
• Use the dual assay (includes secreted luciferase and secreted alkaline phosphatase) to monitor two promoters simultaneously
• Use the dual assay to monitor one promoter with a built-in normalization control

Additional Product Information

Please refer to the product’s Certificate of Analysis for details regarding storage conditions, product components, and technical specifications.
The Kit Components List provides information on included reagents and materials.
Both documents can be found under the Documents tab on the product page.