Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (1D11), Functional Grade, eBioscience

Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (1D11), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 615
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

Description:
The 1D11 monoclonal antibody reacts with human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, γδ T cells, CD8+ T cells, and some CD4+ T cells. NKG2D forms complexes with DAP10 and can co-stimulate multiple NK activation receptors. The counter-receptor for human NKG2D is MICA/MICB, expressed on epithelial tumors of lung, breast, kidney, ovary, prostate, and colon carcinoma.
5C6 and 1D11 block binding of soluble MICA to γδ TCR T cell clones and inhibit lysis by these cells. They induce NKG2D function for redirected lysis of Fc receptor-bearing P815 cells.

Applications Reported:

• Flow cytometric analysis
• Immunoprecipitation
• Immunohistochemical staining
• Inhibition of ligand binding in in vitro assays
  (Fluorochrome-conjugated 1D11 recommended for flow cytometry.)

Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells.
Use ≤0.06 µg/test (defined as staining a cell sample in 100 µL).
Cell number may range from 10⁵ to 10⁸ cells/test; titrate for optimal performance.

Storage and Handling:
Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

Natural killer (NK) cells mediate lysis of tumor and virus-infected cells without prior activation. They regulate humoral and cell-mediated immunity and express several calcium-dependent (C-type) lectins involved in NK cell function.
The NK gene encodes a member of the NKG2 family, a transmembrane protein with type II membrane orientation and a C-type lectin domain. The NKG2 gene family is located within the NK complex region containing several NK-specific lectin genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.