Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (8B.2C12), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 8B.2C12 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein. CD366 is a type I transmembrane protein containing an immunoglobulin and a mucin-like domain extracellularly, and a tyrosine phosphorylation motif intracellularly. It is selectively expressed by differentiated CD4+ Th1 and CD8+ Tc1 cells, but absent on Th2 and Tc2 cells. Other hematopoietic cells such as naive T cells, B cells, macrophages, and dendritic cells do not express CD366 at the protein level.

Expression of CD366 increases at a late stage of Th1 cell differentiation, suggesting a role in effector function rather than differentiation. In an EAE model, CD366 is expressed on most CD4+ and CD8+ T cells in the CNS at disease onset, while peripheral expression remains low. Administration of 8B.2C12 in vivo induces a hyperacute disease phenotype, implying CD366 engagement enhances macrophage activation and autoimmune severity. The Tim gene family is implicated in autoimmunity and allergy regulation.

The 8B.2C12 antibody binds strongly to the BALB/c allele of CD366, with weaker reactivity to the C57Bl/6 allele.

Applications Reported: Flow cytometric analysis and functional studies.
Applications Tested: Tested on mouse splenocytes and Tim-3 transfected cells; optimal ≤ 0.5 µg/test (100 µL final volume). Cell count range: 10⁵–10⁸ cells/test.

Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2)
A 281 amino acid, type I Th1-specific glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. It contains an IgV-like domain, mucin-like domain, and a conserved tyrosine phosphorylation motif. TIM3 is involved in macrophage activation and autoimmune induction, downregulating Th1-mediated responses and promoting immune tolerance. Pathologically, TIM3 contributes to the severity of experimental autoimmune encephalomyelitis (EAE) in mice.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.