Thermo Fisher Scientific CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), Functional Grade, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugate Formats

• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

Description:
The DTA-1 monoclonal antibody reacts with mouse GITR (Glucocorticoid-Induced TNFR family gene, TNFRSF18). In naive mice, GITR is expressed mainly by CD4⁺CD25⁺ T regulatory (Treg) cells and CD25⁺ CD4⁺ CD8⁻ thymocytes. Stimulation with DTA-1 abrogates Treg-mediated suppression. While it does not deplete CD357-expressing cells in vivo, it can induce tumorogenesis. Removal of GITR-expressing Treg cells or DTA-1 administration can lead to organ-specific autoimmune disease.

Applications Reported:

• Flow cytometric analysis
• Immunohistochemistry (frozen mouse tissue)
• Functional assays

Applications Tested:

• Flow cytometry: ≤ 0.03 µg/test (in 100 µL final volume, 10⁵–10⁸ cells/test)
• IHC (Frozen): ≤ 10 µg/mL
  Antibody titration recommended for optimal performance.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90% (SDS-PAGE)

Endotoxin Level:
<0.001 ng/µg antibody (LAL assay)

Aggregation:
<10% (HPLC)

Target Information

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also known as GITR or AITR, is encoded by the TNFRSF18 gene located on chromosome 1p36.33. It belongs to the TNF-receptor superfamily and is upregulated upon T-cell activation. This receptor is critical for maintaining immunological self-tolerance via CD25⁺CD4⁺ regulatory T cells. Mouse knockout studies indicate a role in regulating CD3-driven T-cell activation and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.