Thermo Fisher Scientific RAB5A Monoclonal Antibody (2E8B11), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2E8B11

Immunogen

Purified recombinant fragment of human Rab5a (AA: 1-215) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAB5,uniProtId:P20339-1,ncbiNodeId:9606,antigenRange:1-215,antigenLength:215,antigenImageFileName:14-9711-82_RAB5_P20339-1_House_mouse.svg,antigenImageFileNamePDP:14-9711-82_RAB5_P20339-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2884043

Product Specific Information

Description: This 2E8B11 monoclonal antibody recognizes human Rab5a, which is a GTPase involved in intracellular membrane trafficking. Rab5a is reported to have ubiquitous expression.

Applications Reported: The 2E8B11 clone has been reported for use in intracellular flow cytometry, western blot, ELISA, immunocytochemistry of cells and immunohistochemistry of tissue.

Applications Tested: This 2E8B11 antibody has been tested by western blot in multiple human cell lines.
Applications Tested: This 2E8B11 antibody has been tested by immunocytochemical staining of fixed and permeabilized HeLa cells.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

RAB5 are among the small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.