Thermo Fisher Scientific GFP Recombinant Rabbit Monoclonal Antibody (SP069-0)
GFP 단백질 검출용 항체로 Western blot, IHC, ICC, IP 등 다양한 응용에 적합. Rabbit IgG 기반의 재조합 단클론 항체로 높은 특이성과 재현성 제공. 단백질 A 정제, 액상 형태로 제공되며 장기 보관 시 -20°C 권장. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:1,000 |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SP069-0 |
| Immunogen | Recombinant full length protein of Aequorea victoria GFP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 0.05% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2931490 |
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP is widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic systems, and as a protein tag in cell culture and multicellular organisms. As a fusion tag, GFP enables localization studies, protein movement tracking, and research on subcellular compartment dynamics.
GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (maximally at 395 nm) and emits green light (peak emission at 508 nm) without the need for additional proteins or cofactors. GFP fluorescence is stable, species-independent, and suitable for various applications such as monitoring gene expression, protein localization, studying protein-protein interactions (yeast two-hybrid system), and measuring distances between proteins in FRET experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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