Thermo Fisher Scientific Alpha-Smooth Muscle Actin Recombinant Rabbit Monoclonal Antibody (SY25-03)
Recombinant rabbit monoclonal antibody targeting alpha-smooth muscle actin. Validated for WB, IHC, Flow Cytometry, and IP. High specificity and sensitivity with HEK293 expression system. Suitable for human, mouse, and rat samples. For research use only.
- 카탈로그번호
- MA545033
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY25-03 |
| Immunogen | Synthetic peptide within N-terminal human alpha smooth muscle actin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2931487 |
Target Information
Smooth Muscle Actin belongs to the actin family of highly conserved proteins involved in cell motility, structure, and integrity. Alpha actin is a major component of the contractile apparatus, while beta and gamma actins regulate cell motility. Smooth muscle actin is an alpha actin found in skeletal muscle and smooth muscle vessel walls, gut wall, myometrium, and myoepithelial cells. It plays a role in muscle contraction, cytoskeletal organization, and cell motility. Defects in the smooth muscle actin gene are associated with aortic aneurysm familial thoracic type 6. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells and appears in stress fibers of fibroblastic cells during wound healing and fibrocontractive diseases. Multiple alternatively spliced variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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