Thermo Fisher Scientific Catenin alpha-1 Recombinant Rabbit Monoclonal Antibody (SC0619)
Catenin alpha-1 단백질을 인식하는 recombinant rabbit monoclonal 항체로, Western blot, IHC, ICC, Flow cytometry에 적합합니다. 높은 특이성과 민감도를 제공하며, 로트 간 일관성과 동물 유래 성분이 없는 제형으로 연구용에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC0619 |
| Immunogen | Recombinant protein within Human alpha 1 Catenin (aa 1–90) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809503 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Catenin alpha-1 associates with the cytoplasmic domain of various cadherins to form complexes linked to the actin filament network, crucial for cadherin-mediated cell adhesion. It can associate with both E- and N-cadherins. While initially thought to mediate linkage to the actin cytoskeleton, later studies suggest it does not bind F-actin directly in the complex, implying a different linkage mechanism. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex. It plays an important role in cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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