
Thermo Fisher Scientific LC3B Polyclonal Antibody, DyLight 650
DyLight 650 형광 표지된 LC3B 폴리클로날 항체로 자가포식 관련 단백질 검출에 적합. Western blot, IHC, ICC 등 다양한 응용 가능. Rabbit IgG 기반이며 인간 및 여러 종과 반응. 항원 친화 크로마토그래피로 정제되어 높은 특이성 제공.
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Thermo Fisher Scientific LC3B Polyclonal Antibody, DyLight 650
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
| ELISA | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Avian, Bacteria, Bovine, Dog, Chicken, Guinea pig, Hamster, Human, Invertebrate, Mouse, Non-human primate, Pig, Rabbit, Reptile, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1–100). |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 0.77 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154818 |
Product Specific Information
- This antibody has 92% homology with mouse, 91% with bovine, and 84% with xenopus.
- Reacts weaker with mouse than with human samples.
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) is encoded by the MAP1LC3B gene in humans.
LC3B is associated with microtubule assembly and plays an important role in neurogenesis and autophagy.
It participates in macroautophagy, the major pathway for cytoplasmic component turnover, especially under nutrient starvation.
LC3B mediates interactions between microtubules and cytoskeletal components and is implicated in cancer, aging, metabolic, neurodegenerative, and cardiovascular/pulmonary diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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