Thermo Fisher Scientific LC3B Polyclonal Antibody, DyLight 488
DyLight 488 형광 표지된 LC3B 폴리클로날 항체로 자가포식 관련 단백질 검출에 적합. Western blot, IHC, ICC, Flow 등 다양한 응용 가능. 인체 LC3B 단백질 N-말단 펩타이드로부터 제작되어 높은 종 간 반응성 보유. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
| ELISA | Assay-Dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Avian, Bacteria, Bovine, Dog, Chicken, Guinea pig, Hamster, Human, Invertebrate, Mouse, Non-human primate, Pig, Rabbit, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an N-terminal portion of human LC3 (residues 1–100) |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 0.94 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11156410 |
Product Specific Information
- This antibody has 92% homology with mouse, 91% with bovine, and 84% with xenopus.
- Reacts weaker with mouse than with human samples.
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans is encoded by the MAP1LC3B gene. LC3B is associated with microtubule assembly and plays an important role in neurogenesis.
Studies indicate LC3B is vital in autophagy, a process responsible for degradation of cytoplasmic components.
Three human LC3 isoforms undergo post-translational modifications during autophagy.
Macroautophagy is the major inducible pathway for turnover of cytoplasmic constituents and degradation of enzymes and organelles during nutrient starvation.
LC3B mediates interactions between microtubules and cytoskeletal components and may be involved in cancer, aging, metabolic, neurodegenerative, and cardiovascular/pulmonary diseases.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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