Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio64DEC17), PE, eBioscience

Applications

Immunocytochemistry (ICC/IF)

• Tested dilution: Not specified
• Publications: 2

Flow Cytometry (Flow)

• Tested dilution: 5 µL (0.25 µg)/test
• Publications: 68

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 563
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 605
• Brilliant Violet 786
• eFluor 450
• eFluor 506
• eFluor 660
• FITC
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Custom conjugation available

Product Specific Information

The eBio64DEC17 antibody reacts with human IL-17A and functions as a neutralizing antibody.
Interleukin-17A (IL-17A) is a CD4⁺ T cell-derived cytokine that promotes inflammatory responses and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.

Human IL-17A exists as a glycosylated 20–30 kDa homodimer and induces expression of ICAM-1 in fibroblasts. It also stimulates epithelial, endothelial, and fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In fibroblasts, IL-17A supports CD34⁺ progenitor proliferation and neutrophil maturation. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating cross-species receptor recognition.

IL-23-dependent, IL-17A-producing CD4⁺ T cells (Th17 cells) represent a unique subset distinct from Th1 and Th2 pathways. IFN-γ and IL-4 negatively regulate Th17 differentiation. Intracellular staining by eBio64DEC17 identifies the same population as eBio64CAP17.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated for intracellular staining and flow cytometry of stimulated human peripheral blood cells. Recommended usage: 5 µL (0.25 µg)/test (100 µL final volume).
Excitation/Emission: 488–561 nm / 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a 32 kDa disulfide-linked homodimer cytokine produced by CD4⁺ T cells. It is part of the IL-17 family (IL-17A–F) and signals through IL-17R/CDw217. IL-17A regulates NF-κB and MAPK pathways, induces IL-6 and COX-2 expression, and enhances nitric oxide production. Elevated IL-17A levels are associated with autoimmune and inflammatory diseases such as rheumatoid arthritis, asthma, multiple sclerosis, and psoriasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.