Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio64CAP17), PE, eBioscience
인간 IL-17A를 인식하는 중화능 마우스 단클론 항체로, PE 형광표지되어 있습니다. Flow cytometry, ELISA, ELISPOT, IHC(F) 등 다양한 응용에 적합합니다. 인간 및 원숭이 IL-17A에 반응하며, 염증성 질환 연구에 유용합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio64CAP17), PE, eBioscience
Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio64CAP17), PE, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | 2 Publications |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | 53 Publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Cynomolgus monkey, Human, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio64CAP17 |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1272166 |
Available Formats
- Unconjugated (Cat. 14-7178-81)
- eFluor 660 (Cat. 50-7178-42)
- Functional Grade (Cat. 16-7178-81)
- Custom conjugation available upon request
Product Specific Information
The eBio64CAP17 antibody reacts with human IL-17A and cross-reacts with rhesus monkey IL-17A, verified by intracellular staining. It is a neutralizing antibody with no reactivity to human IL-17F. Reactivity to other IL-17 family members has not been evaluated.
IL-17A (Interleukin-17A) is a CD4⁺ T cell-derived cytokine that promotes inflammatory responses and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. It exists as a glycosylated 20–30 kDa homodimer produced by activated peripheral blood CD4⁺ T cells. IL-17A enhances ICAM-1 expression in fibroblasts and induces IL-6, IL-8, G-CSF, and PGE₂ secretion in epithelial, endothelial, and fibroblastic cells.
Th17 cells, a subset of CD4⁺ T cells, produce IL-17A under IL-23-dependent conditions. IL-17A, IL-17F, and IL-17A/F heterodimers differ in potency for GRO-α secretion, with IL-17A being the most potent.
The eBio64CAP17 antibody can detect IL-17 heterodimers by immunoprecipitation followed by immunoblotting with anti-IL17F monoclonal antibody H17F10A7. It has been shown to react with rhesus and marmoset primates.
Applications Reported:
- Capture antibody in human IL-17A ELISA and ELISPOT assays
- Neutralization of IL-17A bioactivity
- Intracellular staining and flow cytometric analysis of IL-17A-producing cells
Applications Tested:
Pre-titrated for flow cytometry at 5 µL (0.25 µg) per test. One test corresponds to staining a cell sample in 100 µL final volume (10⁵–10⁸ cells/test, empirically determined).
Optical Properties:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a 32 kDa disulfide-linked homodimer (136 amino acids) that signals through IL-17R (CDw217). It regulates NF-κB and MAPK pathways, stimulates IL-6 and COX-2 expression, and enhances NO production. IL-17A supports proliferation of CD34⁺ hematopoietic progenitors and induces neutrophil maturation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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