Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), PE, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 0.25 µg/test
• Publications: View 75 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC-eFluor 780
• Brilliant UV 737
• Brilliant UV 805
• eFluor 450
• eFluor 660
• Functional Grade
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells and antigen-primed CD8 T cells. It is involved in allergic inflammation, parasitic clearance, and tumor-related immune modulation. In humans, IL-13 contributes to B cell isotype switching and downregulates macrophage activity by reducing pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12).

Applications Reported:

• Useful for ELISA and ELISPOT capture
• Suitable for intracellular staining in flow cytometry

Applications Tested:

• Tested for intracellular staining of cultured mouse splenocytes
• Recommended ≤0.25 µg/test (in 100 µL final volume)
• Optimal antibody concentration should be titrated per assay
• Applicable cell count: 10⁵–10⁸ cells/test

Fluorescence Properties:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Filtration:

• 0.2 µm post-manufacturing filtered

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, regulating inflammatory and immune responses. It down-regulates macrophage activity, inhibits pro-inflammatory cytokine production, and contributes to allergen-induced asthma independent of IgE and eosinophils. IL-13 also influences B cell maturation, up-regulates CD23 and MHC class II, and promotes IgE isotype switching.
The IL-13 gene is located on chromosome 5q within a cytokine gene cluster alongside IL3, IL4, IL5, and CSF2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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