Thermo Fisher Scientific IL-9 Polyclonal Antibody, HRP
Human IL-9을 특이적으로 인식하는 HRP 결합 폴리클로날 항체로, WB, IHC, ELISA 등 다양한 면역분석에 적합. 정제된 형태로 높은 민감도와 특이성을 제공하며, 장기 보관 시 -20°C에서 안정적 유지.
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Applications
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | - |
| Immunohistochemistry (IHC) | 1:500–1:2,500 | - |
| ELISA | 1:10,000–1:50,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant human IL-9 protein. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
- This purified antibody has been heated to 56°C for 30 minutes.
- Recognizes both native and recombinant human IL-9 in cell supernatants and certain body fluids.
- A control of similarly diluted normal rabbit IgG is recommended.
Target Information
Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes.
IL-9 regulates cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells.
The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein (~14 kDa).
Native mouse IL-9 is heavily glycosylated.
TGF beta plays an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, driving Th9 cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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