Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Brilliant Ultra Violet 805, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 661, 737
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 510, 555, 585, 610-30S, 660-40S, 800
• NovaFluor Yellow 570, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, interfollicular macrophages, and some dendritic cells. CD14 binds LPS-LBP complexes with high affinity.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Brilliant Ultra Violet™ 805 (BUV805):

• Emission: 797 nm
• Excitation: 355 nm (UV laser required)
• Sensitive to photo-induced oxidation; protect from light

Buffer Recommendations:

• Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409-75) when using multiple polymer dye-conjugated antibodies.

Fixation:

• Compatible with IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark.
• Compatible with short-term methanol-based fixation (≤1 hour).

Laser: Ultraviolet (355 nm)
Emission: 797 nm

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein expressed on monocytes, macrophages, and neutrophils. It functions as a lipopolysaccharide receptor, binding LPS in association with LBP. Soluble CD14 (sCD14) can discriminate structural differences in LPS and contributes to neutralization of serum lipopolysaccharides. CD14 also binds apoptotic cells and influences allergic, inflammatory, and infectious processes. Alternative splicing yields multiple transcript variants encoding the same isoform. CD14 dysfunction is associated with mycobacterium chelonae infection and croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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