Thermo Fisher Scientific Estrogen Receptor alpha Monoclonal Antibody (EVG F9)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA3-310 detects estrogen receptor alpha in human and rabbit samples.
It has been successfully used in immunohistochemistry, immunoprecipitation, and Western blot procedures.
By Western blot, this antibody detects a ~68 kDa protein representing estrogen receptor alpha in MCF-7 cells, human breast tumors, and rabbit vaginal tissue.
In immunohistochemistry, this antibody recognizes the estrogen receptor alpha in MCF-7 cells, human breast tissue, and human smooth muscle cells.

The immunogen is a mix of synthetic peptides corresponding to residues T(140)-S(154) and E(247)-R(263) of human ER alpha.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors.
The estrogen receptor is a ligand-activated transcription factor that, when bound to estrogen, undergoes a conformational change allowing dimerization and binding to estrogen response elements (ERE) in DNA.
When bound to EREs, ER can positively or negatively regulate gene transcription through recruitment of coactivator or corepressor proteins.

There are two forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2).
At least four ERα isoforms are known (66, 53, 47, and 35 kDa).
Estrogen receptors are widely expressed in various tissues, essential for sexual development and reproductive function, and also play roles in bone and other tissues.
They are involved in pathological processes such as breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.