Thermo Fisher Scientific CD337 (NKp30) Monoclonal Antibody (AF29-4D12), eFluor 450, eBioscience
인간 및 비인간 영장류 CD337(NKp30)을 인식하는 단클론 항체로, eFluor 450 형광체 결합. NK 세포 활성화 및 종양/바이러스 감염 세포 분석에 적합. 유세포분석(Flow Cytometry)용으로 사전 적정됨. Violet laser(405 nm)에서 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate, Rhesus monkey |
| Published species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AF29-4D12 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574058 |
Available Formats:
Product Specific Information
This AF29-4D12 monoclonal antibody reacts with human CD337 (NKp30), a major natural cytotoxicity receptor (NCR) expressed on NK cells. CD337 is involved in activating and expanding resting NK cells through interactions with dendritic cells, enabling elimination of tumor, virus-infected, and immature dendritic cells. Reported ligands include B7-H6.
The AF29-4D12 antibody cross-reacts with rhesus macaque.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells
- Recommended use: 5 µL (0.125 µg) per test (100 µL final volume)
- Suitable for 10⁵–10⁸ cells/test
Fluorochrome Information:
- eFluor® 450 is an alternative to Pacific Blue®
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that assists NK cells in lysing tumor cells. It interacts with CD3-zeta (CD247), a T-cell receptor component. A polymorphism in the 5′ untranslated region has been associated with mild malaria susceptibility. Three transcript variants encoding different isoforms have been identified (RefSeq, May 2010).
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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