Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), eFluor 450, eBioscience
마우스 NKp46(CD335)을 인식하는 Rat IgG2a 단클론 항체로 eFluor 450 형광표지. NK 세포 특이적 마커로 Flow Cytometry에 적합하며, 405/445 nm에서 검출 가능. 비동결 보관, in vitro 활성화 가능.
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Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 19 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 29A1.4 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10548521 |
Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- eFluor 660
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The monoclonal antibody 29A1.4 recognizes mouse NKp46 (CD335), a member of the natural cytotoxicity receptor (NCR) family. CD335 is a glycoprotein with two Ig-like domains and a short cytoplasmic tail, expressed uniquely on NK cells, including immature NK cells (DX5- CD3-), allowing discrimination between NKT and NK cells (NKp46+, CD3-).
Unlike many NK markers that also stain NKT cells, 29A1.4 staining is not strain-specific and has been confirmed on C57Bl/6, SJL, CBA/CA, and BALB/C strains. NKp46 plays a role in NK cell-mediated lysis of tumor and pathogen-infected cells.
The 29A1.4 antibody activates NK cells in vitro but does not deplete NK cells in vivo.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Cell number can range from 10⁵–10⁸ cells/test. Optimize titration for best performance.
eFluor® 450 is an alternative to Pacific Blue®, excited by violet laser (405 nm) and emits at 445 nm. Ensure instrument compatibility.
Excitation: 405 nm
Emission: 445 nm
Laser: Violet Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor contributing to NK cell-mediated tumor cell lysis. It consists of two Ig-like domains similar to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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