Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), eFluor 450, eBioscience
Thermo Fisher Scientific의 Arginase 1 Monoclonal Antibody (A1exF5), eFluor 450은 인간 및 마우스 Arginase 1 검출용 항체로, Flow Cytometry에 최적화되어 있습니다. eFluor 450 형광체를 사용하며, 405/445 nm 파장에서 검출 가능합니다. 세포 내 단백질 염색에 적합하고 높은 특이성과 재현성을 제공합니다.
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Applications
Immunohistochemistry (IHC)
- Tested Dilution: -
- View 1 publication
Immunocytochemistry (ICC/IF)
- Tested Dilution: -
- View 1 publication
Flow Cytometry (Flow)
- Tested Dilution: 1.0 µg/test
- View 21 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host/Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A1exF5 |
| Immunogen | E.coli-derived recombinant mouse Arginase 1 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2734836 |
Additional Formats
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 650
- Brilliant Violet 711
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1 (Arg1), a cytosolic enzyme.
This clone is compatible with standard intracellular staining protocols and the Foxp3/Transcription Factor Staining Buffer Set.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00)
- Recommended protocol: BestProtocols® – Protocol A: Two-step protocol for cytoplasmic intracellular proteins
Use ≤ 1.0 µg per test (100 µL final volume). Cell number can range from 10⁵ to 10⁸ cells/test. Antibody titration is recommended for optimal performance.
eFluor™ 450 is an alternative to Pacific Blue™.
- Emission: 446 nm
- Excitation: 405 nm
- Laser: Violet laser
Ensure instrument compatibility with this fluorochrome.
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, the final step in the urea cycle.
It plays a role in cell proliferation and detoxification. By degrading arginine, Arginase 1 down-regulates nitric oxide production.
- Expression: Liver, neutrophils, myeloid-derived suppressor cells (MDSC), neural stem cells
- Human: Expressed in blood neutrophils but not CCR3+ granulocytes
- Mouse: Marker of alternatively activated macrophages (M2a)
- Clinical relevance: Found in hepatocellular carcinoma and myeloid tumor-infiltrating cells
- Defects: Cause of argininemia (autosomal recessive disorder with hyperammonemia)
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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