Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, eFluor 450, eBioscience

Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, eFluor 450

Applications and Tested Dilution

• Flow Cytometry (Flow): 0.5 µg/test

Product Specifications

Additional Formats Available

• APC
• Biotin
• eFluor 570
• eFluor 615
• eFluor 660
• FITC
• HRP
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Custom conjugation available upon request

Product Specific Information

The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and serves as a secondary reagent in flow cytometry and microscopy. It does not recognize other mouse isotypes or rat immunoglobulins.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric staining with Mouse IgG1 primary antibody
• Recommended usage: ≤ 0.5 µg per test (100 µL final volume)
• Cell number range: 10⁵–10⁸ cells/test
• Optimize antibody titration for best performance

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm. Compatible with violet laser instruments.

Filtration: 0.2 µm post-manufacturing filtered

Target Information

Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins. They are used for detection, sorting, or purification of target molecules. Secondary antibodies enhance assay versatility and sensitivity via signal amplification.

These antibodies are typically produced by immunizing host animals with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, or conjugation).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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