Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, eFluor 450, eBioscience

Applications

Tested Dilution

Flow Cytometry (Flow)

0.5 µg/test

Product Specifications

Host/Isotype

Rat / IgG

Class

Monoclonal

Type

Secondary Antibody

Clone

M1-14D12

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

View additional formats

• [APC ](/antibody/secondary/product-name/Mouse IgG1/conjugates/APC/host/Rat/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG1/conjugates/Biotin/host/Rat/)
• [eFluor 570](/antibody/secondary/product-name/Mouse IgG1/conjugates/eFluor 570/host/Rat/)
• [eFluor 615](/antibody/secondary/product-name/Mouse IgG1/conjugates/eFluor 615/host/Rat/)
• [eFluor 660](/antibody/secondary/product-name/Mouse IgG1/conjugates/eFluor 660/host/Rat/)
• [FITC](/antibody/secondary/product-name/Mouse IgG1/conjugates/FITC/host/Rat/)
• [HRP](/antibody/secondary/product-name/Mouse IgG1/conjugates/HRP/host/Rat/)
• [PE ](/antibody/secondary/product-name/Mouse IgG1/conjugates/PE/host/Rat/)
• [PE-Cyanine7](/antibody/secondary/product-name/Mouse IgG1/conjugates/PE-Cyanine7/host/Rat/)
• [PE-eFluor 610](/antibody/secondary/product-name/Mouse IgG1/conjugates/PE-eFluor 610/host/Rat/)
• [PerCP-eFluor 710](/antibody/secondary/product-name/Mouse IgG1/conjugates/PerCP-eFluor 710/host/Rat/)
• [Super Bright 436](/antibody/secondary/product-name/Mouse IgG1/conjugates/Super Bright 436/host/Rat/)
• [Super Bright 600](/antibody/secondary/product-name/Mouse IgG1/conjugates/Super Bright 600/host/Rat/)
• [Super Bright 780](/antibody/secondary/product-name/Mouse IgG1/conjugates/Super Bright 780/host/Rat/)
• Request custom conjugation

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2574059

Target

IgG1

Antibody Form

Whole Antibody

Product Specific Information

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.

Applications Reported: The 1C10 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of staining with a mouse IgG1 primary antibody. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.