Thermo Fisher Scientific B7-H4 (Immuno-Inhibitory Protein) Monoclonal Antibody (B7H4, 1788)
B7-H4 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow Cytometry에 사용 가능. 인간 시료에 반응하며, 단백질 억제 기능 연구 및 암 면역조절 연구에 적합. 단백질 A/G 정제, PBS 용액 형태로 제공.
- 카탈로그번호
- 79679-MSM1-P1ABX
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific B7-H4 (Immuno-Inhibitory Protein) Monoclonal Antibody (B7H4, 1788)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B7H4, 1788 |
| Immunogen | Recombinant fragment of human B7-H4 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C or −80°C (preferred) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Tested in direct ELISA (use antibody at 2–4 µg/mL for coating).
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
B7-H4 (B7x, VTCN1) is a costimulatory protein functioning as a negative regulator of T-cell mediated immunity.
Although its receptor is unknown, B7-H4 is believed to deliver an inhibitory signal to T-cells, suppressing proliferation, cell cycle progression, and interleukin-2 production.
B7-H4 deficient mice show minimal immune alteration, suggesting its role in fine-tuning immune responses.
It is expressed on activated T-cells, B-cells, monocytes, and dendritic cells. Aberrant expression is linked to cancers of the lung, breast, and ovary.
B7-H4 is primarily located on lymphoid cell membranes and acts as an immunoinhibitory protein interacting with T lymphocyte receptors. Overexpression may aid tumor cells in evading immune detection, particularly in ovarian and breast cancers.
VTCN1 may serve as a potential biomarker for early detection of ovarian cancer and renal cell carcinoma (RCC).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.