Thermo Fisher Scientific B7-H4 (Immuno-Inhibitory Protein) Monoclonal Antibody (B7H4, 1788)
B7-H4 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 인간 시료에 반응하며, Protein A/G 정제 및 PBS 완충액에 보관. T세포 면역 조절 관련 연구에 적합.
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- 79679-MSM1-P1
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B7H4, 1788 |
| Immunogen | Recombinant fragment of human B7-H4 protein (amino acids 32–147) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed samples): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
B7-H4 (also known as B7x, VTCN1) is a co-stimulatory protein functioning as a negative regulator of T-cell mediated immunity. It delivers inhibitory signals to T-cells, preventing proliferation, cell cycle progression, and interleukin-2 production.
B7-H4 is expressed on activated T-cells, B-cells, monocytes, and dendritic cells. Aberrant expression has been linked to cancers of the lung, breast, and ovary.
Overexpression may allow tumor cells to suppress T-cell immunity and promote tumor progression. VTCN1 is also considered a potential biomarker for early detection of ovarian cancer and renal cell carcinoma (RCC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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