Thermo Fisher Scientific Opsin 4 Polyclonal Antibody
Opsin 4 단백질을 검출하기 위한 Thermo Fisher Scientific의 다클론 항체로, 마우스 및 랫트 반응성. Western blot, IHC, ICC/IF 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 1 mg/mL 액상 형태. -20°C 보관, 동결·해동 반복 피할 것.
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Thermo Fisher Scientific Opsin 4 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–4 µg/mL | View 5 publications |
| Immunohistochemistry (IHC) | – | View 16 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 2 publications |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | View 12 publications |
| Immunomicroscopy (IM) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Fruit fly, Hamster, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues K M(1) N S P S E S R V P S S L T Q D P S F(19) of rat melanopsin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2267547 |
Product Specific Information
PA1-780 detects mouse and rat melanopsin.
It has been successfully used in Western blot, immunofluorescence, and immunocytochemistry procedures.
By Western blot, this antibody detects ~53 and ~85 kDa proteins representing overexpressed unglycosylated and glycosylated melanopsin from HEK 293 cells (does not work well on endogenously expressed protein).
Immunofluorescence and immunocytochemistry staining of melanopsin in rat retinal ganglion cells (RGCs) can be performed using PA1-780.
The immunogen is a synthetic peptide corresponding to residues K M(1) N S P S E S R V P S S L T Q D P S F(19) of rat melanopsin.
Target Information
Melanopsin is an opsin-like protein found in the retinal ganglion cells of mammals.
It is part of a secondary optical system that parallels the classic rod and cone system.
This system reports directly to the suprachiasmatic nucleus and regulates circadian rhythms.
Melanopsin knockout mice exhibit decreased capacity to entrain to light and dark cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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